Blood Gas Parameters — Normal Ranges
pH
7.35 – 7.45
Acid-base balance
PaCO₂
4.7 – 6.0 kPa
35 – 45 mmHg · Respiratory
PaO₂
10 – 13 kPa
75 – 100 mmHg · Oxygenation
HCO₃⁻
22 – 26 mmol/L
Metabolic component
Base Excess
−2 to +2
mmol/L
SaO₂
> 95%
Haemoglobin O₂ saturation
CO₂ = Acid / HCO₃⁻ = Base
  • ↑ CO₂ → ↓ pH (more acid = acidaemia)
  • ↓ CO₂ → ↑ pH (less acid = alkalaemia)
  • ↑ HCO₃⁻ → ↑ pH (more base = alkalaemia)
  • ↓ HCO₃⁻ → ↓ pH (less base = acidaemia)
  • CO₂ is regulated by the lungs (minutes)
  • HCO₃⁻ is regulated by the kidneys (hours–days)
Acid-Base Definitions
  • Acidaemia: pH < 7.35
  • Alkalaemia: pH > 7.45
  • Acidosis: process causing accumulation of acid / loss of base
  • Alkalosis: process causing loss of acid / accumulation of base
  • Respiratory disorder: primary change in PaCO₂
  • Metabolic disorder: primary change in HCO₃⁻
SaO₂ vs SpO₂ vs PaO₂
ParameterWhat it measuresMethodClinical use
PaO₂Dissolved O₂ in arterial blood (pressure)ABG arterial sampleTrue oxygenation status; not affected by CO poisoning
SaO₂% haemoglobin saturated with O₂ (arterial)ABG co-oximetryAccurate; measures all haemoglobin species
SpO₂Estimated haemoglobin saturation via light absorbancePulse oximeterNon-invasive continuous monitoring; has limitations
  • S-shaped curve — flat upper portion = O₂ loading (lungs), steep lower = O₂ unloading (tissues)
  • P50 = PaO₂ at which Hb is 50% saturated (normal ≈ 3.5 kPa / 26 mmHg)
  • Plateau above ~8 kPa — large fall in PaO₂ needed to significantly reduce SaO₂
  • ↑ Temperature (fever)
  • ↑ PaCO₂ (hypercapnia)
  • ↑ H⁺ / ↓ pH (acidosis) — Bohr effect
  • ↑ 2,3-DPG (chronic anaemia, altitude)
  • ↓ Temperature, ↓ CO₂, ↓ H⁺, ↑ pH, Foetal Hb, CO poisoning
Types of Hypoxia
TypeMechanismExamplePaO₂SaO₂
HypoxaemicLow O₂ in arterial bloodPneumonia, ARDS, Type 1 RF↓ Low↓ Low
AnaemicReduced O₂ carrying capacityHaemorrhage, haemolytic anaemiaNormalNormal
Ischaemic / StagnantReduced blood flow to tissuesCardiogenic shock, heart failureNormalNormal
HistotoxicCells unable to utilise O₂CO poisoning, cyanide poisoningNormalNormal (falsely)
CO Poisoning Warning SpO₂ will read falsely normal (carboxyhaemoglobin is read as oxyhaemoglobin by standard pulse oximeters). Diagnosis requires co-oximetry on ABG sample. Treat with high-flow 100% O₂.
5-Step Systematic ABG Interpretation
  1. Is the pH normal, acidaemic, or alkalaemic?Normal 7.35–7.45. pH <7.35 = acidaemia; pH >7.45 = alkalaemia. Even if pH is in normal range, a disorder may still be present (compensated).
  2. Look at PaCO₂ — does it explain the pH?If pH ↓ and CO₂ ↑ = respiratory acidosis is primary. If pH ↑ and CO₂ ↓ = respiratory alkalosis is primary. CO₂ same direction as pH change = metabolic primary.
  3. Look at HCO₃⁻ — does it explain the pH?If pH ↓ and HCO₃⁻ ↓ = metabolic acidosis is primary. If pH ↑ and HCO₃⁻ ↑ = metabolic alkalosis is primary.
  4. Is there compensation?Lungs compensate for metabolic disorders (fast). Kidneys compensate for respiratory disorders (slow, 3–5 days). Compensation never fully corrects pH (except metabolic alkalosis sometimes).
  5. Is there a mixed disorder?Compare expected vs actual compensation using formulas. If compensation is inappropriate (too much or too little) = mixed acid-base disorder.
Respiratory Acidosis
↓ pH
↑ CO₂
  • COPD exacerbation (Type 2 failure)
  • Opiate / sedative overdose
  • Neuromuscular weakness (MG, GBS)
  • Severe asthma (exhaustion)
  • Chest wall deformity / obesity hypoventilation
  • Central respiratory depression
  • Kidneys retain HCO₃⁻ → ↑ HCO₃⁻
  • Acute: HCO₃⁻ ↑ 1 mmol/L per 10 mmHg ↑ CO₂
  • Chronic: HCO₃⁻ ↑ 3.5 mmol/L per 10 mmHg ↑ CO₂
Respiratory Alkalosis
↑ pH
↓ CO₂
  • Anxiety / hyperventilation
  • Pain
  • Pulmonary embolism (early)
  • Early salicylate (aspirin) poisoning
  • Mechanical ventilation (over-ventilation)
  • Liver failure / pregnancy
  • Altitude, fever, sepsis
  • Kidneys excrete HCO₃⁻ → ↓ HCO₃⁻
  • Acute: HCO₃⁻ ↓ 2 mmol/L per 10 mmHg ↓ CO₂
  • Chronic: HCO₃⁻ ↓ 5 mmol/L per 10 mmHg ↓ CO₂
Metabolic Acidosis — Anion Gap Analysis
↓ pH
↓ HCO₃⁻
Anion Gap = Na⁺ − (Cl⁻ + HCO₃⁻)  |  Normal: 8–12 mmol/L (up to 16 if albumin not corrected)
M
MethanolToxic alcohol ingestion
U
UraemiaRenal failure (organic acids)
D
DKADiabetic ketoacidosis
P
Propylene glycolMedication solvent
I
Isoniazid / InfectionSepsis (lactic acidosis)
L
Lactic acidosisShock, sepsis, ischaemia
E
Ethylene glycolAntifreeze ingestion
S
SalicylatesLate aspirin poisoning
  • Hyperchloraemia (excess saline)
  • Addison's disease (adrenal insufficiency)
  • Renal tubular acidosis
  • Diarrhoea (HCO₃⁻ loss)
  • Ureteral diversion / fistula
  • Pancreatic fistula
  • Saline infusion (dilutional)
  • Winter's formula: Expected PaCO₂ (mmHg) = (1.5 × HCO₃⁻) + 8 ± 2
  • Kussmaul breathing — deep, sighing respirations in DKA
Metabolic Alkalosis
↑ pH
↑ HCO₃⁻
  • Vomiting / NG suction (H⁺Cl⁻ loss)
  • Thiazide or loop diuretics (K⁺ + H⁺ loss)
  • Primary hyperaldosteronism (Conn's)
  • Excess alkali administration
  • Hypomagnesaemia / hypokalaemia
  • Contraction alkalosis (volume depletion)
  • Hypoventilation → ↑ CO₂ to buffer alkalosis
  • Expected CO₂ (mmHg) = (0.7 × HCO₃⁻) + 21 ± 2
  • Compensation is limited (hypoxic drive limits hypoventilation)
Chloride-Responsive vs Resistant Urine Cl⁻ <20 = chloride-responsive (vomiting/diuretics). Urine Cl⁻ >20 = chloride-resistant (hyperaldosteronism).
Summary — Quick Reference
DisorderpHPrimary changeCompensationCompensatory change
Respiratory Acidosis↓ <7.35↑ CO₂Renal (days)↑ HCO₃⁻
Respiratory Alkalosis↑ >7.45↓ CO₂Renal (days)↓ HCO₃⁻
Metabolic Acidosis↓ <7.35↓ HCO₃⁻Respiratory (mins)↓ CO₂
Metabolic Alkalosis↑ >7.45↑ HCO₃⁻Respiratory (mins)↑ CO₂
Type 1 Respiratory Failure
Hypoxaemic only
PaO₂
< 8 kPa
<60 mmHg
PaCO₂
Normal / ↓ low
≤ 6.0 kPa
  • Pneumonia (V/Q mismatch)
  • Pulmonary oedema (cardiogenic)
  • Pulmonary embolism
  • ARDS (diffuse alveolar damage)
  • Pneumothorax
  • Pulmonary fibrosis (diffusion defect)
  • Pleural effusion
Type 2 Respiratory Failure
Hypoxaemic + Hypercapnic
PaO₂
< 8 kPa
<60 mmHg
PaCO₂
> 6 kPa
>45 mmHg
  • COPD exacerbation (most common in GCC)
  • Severe asthma (with exhaustion/fatigue)
  • Neuromuscular disease (MG, GBS, MND)
  • Chest wall deformity (kyphoscoliosis)
  • Obesity hypoventilation syndrome
  • Opioid / sedative overdose
  • Central respiratory depression
COPD Oxygen Target — SpO₂ 88–92% In Type 2 COPD patients, the hypoxic drive (not the hypercapnic drive, which is blunted) maintains ventilatory effort. High-flow O₂ → corrects hypoxia → removes respiratory drive → hypoventilation → CO₂ retention → CO₂ narcosis. Use controlled oxygen via Venturi mask. Target SpO₂ 88–92% (vs 94–98% in most other patients).
ARDS — Berlin Definition (2012)
SeverityPaO₂/FiO₂ RatioMortality
Mild200 – 300 mmHg~27%
Moderate100 – 200 mmHg~32%
Severe< 100 mmHg~45%
A-a Gradient
A-a gradient = PAO₂ − PaO₂
PAO₂ = (FiO₂ × 713) − (PaCO₂ / 0.8) [mmHg on air: FiO₂ = 0.21]
Normal A-a = Age/4 + 4 mmHg (on room air)
  • Normal A-a: Hypoventilation (PE excluded)
  • Elevated A-a: V/Q mismatch, diffusion defect, right-to-left shunt
  • Useful to distinguish Type 1 causes from pure hypoventilation
NEWS2 & ABG Integration
  • NEWS2 uses SpO₂ Scale 1 (target 96–100%) for most patients
  • NEWS2 uses SpO₂ Scale 2 (88–92%) for confirmed Type 2 failure / COPD at risk of hypercapnia
  • Abnormal ABG in context of rising NEWS2 = escalate immediately
  • GCC ICUs: ABG every 4–6 hours in intubated/ventilated patients as standard
  • Trend matters: deteriorating PaO₂ + rising PaCO₂ = imminent intubation
Radial Artery Puncture — Procedure
  1. Occlude both radial and ulnar arteries simultaneously — hand should blanch
  2. Release ulnar artery only — observe for reperfusion
  3. Normal: colour returns within <7 seconds = adequate collateral circulation = safe to proceed
  4. Abnormal: >15 seconds = inadequate ulnar collateral = choose alternative site
  • Heparinised syringe (1 mL pre-filled)
  • Wrist dorsiflexed 30–45° (rolled towel under wrist)
  • Locate pulse; clean with 2% chlorhexidine
  • 45° angle of needle insertion, bevel up
  • Pulsatile blood flow confirms arterial placement
  • Remove air bubbles immediately (↑ PaO₂, ↓ PaCO₂ if not removed)
  • Apply firm pressure for 5 minutes post-procedure
  • Analyse on ice within 30 min, or room temperature within 30 min
Complications of Arterial Puncture / Lines
ComplicationManagement
ThrombosisUsually resolves spontaneously; monitor perfusion
HaematomaDirect pressure; elevate limb
VasospasmUsually temporary; warm compress
Pseudo-aneurysmUltrasound-guided compression or surgery
InfectionAseptic technique; replace line if SIRS signs
Air embolismPrevention: remove all air before injection
Nerve injuryCareful technique; document neurovascular checks
Arterial Line Nursing Management
  • Zero transducer at the phlebostatic axis (4th intercostal space, mid-axillary line)
  • Zero with patient supine, transducer at heart level
  • Re-zero when patient position changes or readings questioned
  • Open stopcock to air, zero on monitor, close
  • 300 mmHg pressure bag with normal saline (heparin optional)
  • Continuous flush at 1–3 mL/hr to maintain patency
  • Inspect circuit daily: check connections, kinks, air
  • Change complete circuit every 96 hours per local policy
  • Normal: Sharp upstroke, dicrotic notch, clear systolic/diastolic peaks
  • Over-damped: Dampened rounded waveform, falsely low systolic. Check: clots, kinks, air, loose connections. Perform fast-flush test.
  • Under-damped: Systolic overshoot (resonance). Narrow tubing/catheter, hyperdynamic circulation. Use damping device.
  1. Discard / waste 3 mL first (dead space + diluted blood)
  2. Collect sample into heparinised syringe
  3. Flush line; ensure waveform returns
  4. Clearly label sample as ARTERIAL
NEVER inject medication via an arterial line. Intra-arterial injection of drugs (including potassium, antibiotics, anaesthetic agents) causes severe vasospasm and limb-threatening ischaemia. Label ALL arterial lines prominently with RED "ARTERIAL" tape. This is a critical patient safety issue and a common exam topic.
Arterial vs Venous Differentiation Arterial blood is pulsatile, bright red, and flows under pressure. Venous blood is darker, non-pulsatile, and flows slowly. Always confirm placement with waveform before use. Mis-labelling arterial samples as venous (or vice versa) is a significant clinical error.
Capnography — ETCO₂ Monitoring

End-tidal CO₂ (ETCO₂) measures CO₂ at end of exhalation — approximates alveolar and (in normal lungs) arterial PaCO₂

Normal ETCO₂
3.5 – 5.0 kPa
35 – 45 mmHg
  • Phase I (A–B): Inspiratory baseline — dead space gas, CO₂ ≈ 0
  • Phase II (B–C): Upstroke — alveolar gas mixing with dead space, CO₂ rising
  • Phase III (C–D): Alveolar plateau — plateau of CO₂-rich alveolar gas; ETCO₂ read at peak D
  • Phase IV (D–E): Inspiratory downstroke — rapid fall to baseline
  • Hypoventilation (↓ respiratory rate/depth)
  • ↑ CO₂ production — sepsis, fever, MH
  • Rebreathing (exhausted soda lime, equipment fault)
  • Obstruction (bronchospasm — shark fin waveform)
  • Hyperventilation
  • Poor circulation / cardiac arrest
  • PE (dead space ventilation — V/Q mismatch)
  • Oesophageal intubation (ETCO₂ ≈ 0)
ETCO₂ During CPR ETCO₂ <1.3 kPa (10 mmHg) after 20 min CPR = poor prognosis. Sudden ↑ ETCO₂ during CPR = indicator of ROSC (return of spontaneous circulation).
Pulse Oximetry — Principles & Limitations
  • Uses two wavelengths of light (660 nm red; 940 nm infrared)
  • Oxyhaemoglobin and deoxyhaemoglobin absorb light differently
  • Pulsatile component (AC) separated from non-pulsatile (DC)
  • SpO₂ calculated from ratio of AC/DC at each wavelength
ConditionTarget SpO₂
Most acutely unwell patients94 – 98%
COPD / Type 2 RF risk88 – 92%
Post-cardiac arrest94 – 98% (avoid hyperoxia)
Preterm neonates91 – 95%
Palliative / comfortGuided by symptom relief
CauseEffect on SpO₂
Motion artefactFalsely low / erratic readings
Peripheral vasoconstriction (hypothermia, shock)Poor signal / falsely low
Nail varnish (blue, black, green)Falsely low
Dark skin pigmentationMay overread SpO₂ by 2–4%
CO poisoningFalsely NORMAL (COHb read as OxyHb)
MethaemoglobinaemiaReads approx 85% regardless of true value
Severe anaemia (Hb <5 g/dL)May be inaccurate
JaundiceMinimal effect with modern devices
GCC ICU Standard Practice In ventilated patients in GCC ICUs, both continuous SpO₂ AND continuous ETCO₂ monitoring are standard of care. ETCO₂ confirms endotracheal tube position and provides early warning of haemodynamic deterioration. Waveform capnography is mandatory for intubated patients per DHA/DOH standards.
GCC-Specific Clinical Context
  • COPD / Asthma: High prevalence — smoking rates (especially male), sandstorm exposure (PM2.5/PM10), occupational exposures (construction, oil/gas). COPD Type 2 failure management is a core competency.
  • Diabetes (Type 2): Highest prevalence globally in Gulf states. DKA is the most common ABG emergency — metabolic acidosis with high anion gap.
  • Obesity: High rates of obesity hypoventilation syndrome (OHS) — Type 2 respiratory failure, polycythaemia.
  • Heat-related illness: Hyperventilation, respiratory alkalosis; lactic acidosis in severe heatstroke.
ParameterExpected change
pH↓ Low (<7.35, often <7.1 in severe)
PaCO₂↓ Low (Kussmaul compensation)
HCO₃⁻↓ Very low (<15 mmol/L)
Anion gap↑ High (>12, often >20)
K⁺Often ↑ (but total body K⁺ ↓)
Glucose↑ >11 mmol/L (usually >14)
Kussmaul Breathing Deep, sighing, rapid respirations — respiratory compensation for metabolic acidosis in DKA. Not to be confused with Cheyne-Stokes (CNS/HF) or Biot's (brainstem) breathing.

⚙ Interactive ABG Interpreter

Enter blood gas values to receive a complete systematic interpretation, compensation analysis, and clinical guidance.

DHA / DOH / SCFHS Exam Practice — 10 MCQs

Click "Show Answer" after selecting your option. These question styles are representative of GCC licensing examinations.

1A patient has pH 7.28, PaCO₂ 7.8 kPa, HCO₃⁻ 28 mmol/L. What is the primary disorder?
  • A. Metabolic acidosis
  • B. Respiratory acidosis with metabolic compensation
  • C. Mixed respiratory and metabolic acidosis
  • D. Metabolic alkalosis
B. The pH is low (acidaemia). PaCO₂ is elevated — this drives the acidosis (respiratory acidosis). HCO₃⁻ is elevated at 28 mmol/L — this is renal compensation (not primary). The elevated HCO₃⁻ is consistent with chronic respiratory acidosis compensation.
2In COPD, why should supplemental oxygen be titrated to achieve SpO₂ 88–92% rather than higher?
  • A. To prevent pulmonary oedema from oxygen toxicity
  • B. High-flow O₂ abolishes hypoxic respiratory drive, causing hypoventilation and CO₂ retention
  • C. Higher O₂ levels cause paradoxical bronchospasm in COPD
  • D. To reduce the risk of oxygen-induced pneumonia
B. Chronic hypercapnia in COPD blunts the normal CO₂ drive to breathe. The hypoxic drive becomes the primary stimulus. Excessive O₂ removes this drive, leading to hypoventilation, CO₂ retention, respiratory acidosis, and CO₂ narcosis.
3A 34-year-old with poorly controlled Type 2 diabetes presents confused. ABG: pH 7.08, PaCO₂ 2.8 kPa, HCO₃⁻ 6 mmol/L, Na 138, Cl 98. What is the anion gap?
  • A. 12 mmol/L (normal)
  • B. 34 mmol/L (high anion gap)
  • C. 8 mmol/L (normal)
  • D. 22 mmol/L (borderline)
B. Anion gap = Na − (Cl + HCO₃⁻) = 138 − (98 + 6) = 34 mmol/L. This is severely elevated, consistent with DKA (ketoacidosis). The very low pH, low HCO₃⁻, and low PaCO₂ (Kussmaul compensation) confirm severe metabolic acidosis with appropriate respiratory compensation.
4A patient's SpO₂ reads 97% but blood gas reveals PaO₂ of 8.5 kPa and the patient appears cyanosed. What is the most likely cause?
  • A. Calibration error in the ABG analyser
  • B. Venous blood was taken instead of arterial
  • C. Carbon monoxide poisoning (carboxyhaemoglobinaemia)
  • D. Methaemoglobinaemia
C. Carboxyhaemoglobin (COHb) absorbs light at similar wavelengths to oxyhaemoglobin, causing pulse oximetry to read falsely normal. ABG with co-oximetry is required. Methaemoglobinaemia would read ~85%, not 97%.
5During CPR, the ETCO₂ suddenly rises from 0.8 kPa to 4.2 kPa. What does this indicate?
  • A. Improved chest compression quality only
  • B. Return of spontaneous circulation (ROSC)
  • C. Oesophageal intubation
  • D. CO₂ accumulation from inadequate ventilation
B. A sudden, sustained rise in ETCO₂ during CPR is a strong indicator of ROSC. When the heart restarts, pulmonary blood flow returns, washing out CO₂ that has accumulated in the tissues. ETCO₂ <1.3 kPa after 20 minutes of CPR is associated with poor prognosis.
6Modified Allen's test is performed before radial artery cannulation. The hand takes 18 seconds to regain colour after releasing the ulnar artery. What is the correct action?
  • A. Proceed with cannulation — 18 seconds is acceptable
  • B. Repeat the test 3 times before deciding
  • C. Do not proceed — inadequate collateral circulation; choose an alternative site
  • D. Perform the procedure but with extra care
C. Normal reperfusion is <7 seconds, indicating adequate ulnar collateral circulation. >15 seconds indicates poor collateral supply — proceeding risks hand ischaemia if radial artery thrombosis occurs. Use femoral, brachial, or dorsalis pedis as alternatives.
7A patient has pH 7.48, PaCO₂ 3.2 kPa, HCO₃⁻ 18 mmol/L. What is the most likely diagnosis?
  • A. Metabolic alkalosis with respiratory compensation
  • B. Acute respiratory alkalosis (hyperventilation)
  • C. Compensated metabolic acidosis
  • D. Mixed alkalosis
B. pH is high (alkalaemia). PaCO₂ is very low — this is the primary driver (respiratory alkalosis). HCO₃⁻ is slightly low at 18 — this represents early/partial renal compensation. The clinical picture suggests acute hyperventilation (anxiety, pain, PE, early sepsis).
8An arterial line waveform appears dampened with a rounded, blunted systolic peak. The patient's blood pressure reads 82/60 but they appear well with good peripheral perfusion. What is the FIRST action?
  • A. Administer a fluid bolus for presumed hypotension
  • B. Check the arterial line circuit — assess for clots, kinks, air, or loose connections
  • C. Call the doctor immediately for urgent review
  • D. Remove the arterial line and replace
B. A dampened waveform is a technical problem, not necessarily a patient problem. Causes include clots in the catheter, air bubbles, kinked tubing, or loose connections. Always troubleshoot the equipment before treating the patient. Perform a fast-flush test to assess dynamic response.
9According to the Berlin definition, a patient on PEEP 8 cmH₂O with bilateral infiltrates has PaO₂/FiO₂ ratio of 150 mmHg. How is their ARDS classified?
  • A. Mild ARDS
  • B. Moderate ARDS
  • C. Severe ARDS
  • D. Does not meet Berlin criteria
B. Berlin Definition severity: Mild = PF ratio 200–300; Moderate = 100–200; Severe = <100 mmHg (all on PEEP ≥5 cmH₂O). PF ratio 150 mmHg on PEEP 8 cmH₂O = Moderate ARDS. Associated mortality approximately 32%.
10A patient with metabolic acidosis has HCO₃⁻ of 12 mmol/L. Using Winter's formula, what is the expected PaCO₂ in mmHg if compensation is appropriate?
  • A. 26 mmHg
  • B. 26 ± 2 mmHg (range 24–28)
  • C. 35 mmHg (normal)
  • D. 18 mmHg
B. Winter's formula: Expected PaCO₂ = (1.5 × HCO₃⁻) + 8 ± 2 = (1.5 × 12) + 8 ± 2 = 18 + 8 ± 2 = 26 ± 2 mmHg (24–28 mmHg). If the measured PaCO₂ is within this range, compensation is appropriate (simple metabolic acidosis). If PaCO₂ is higher than expected = concomitant respiratory acidosis; lower = respiratory alkalosis.